Abstract
ABSTRACTKlebsiella pneumoniae is a promising industrial species for the production of chemicals, yet its opportunistic pathogenicity restrains real-world applications. In this work, we identified a novel isolate designated Klebsiella sp. AA405 which carried low levels of virulence factors. No capsule, fimbriae and flagella were observed around colonies. Genome alignment between Klebsiella sp. AA405 and other 186 Klebsiella species revealed 3421 homologous genes. Notably, the endotoxin of Klebsiella sp. AA405 was only half of E. coli BL21. This strain matched well with common cloning and expression vectors and was sensitive to antibiotics. More importantly, this strain metabolized diverse carbon sources. Under micro-aerobic conditions, it produced 51.66 g/L 1,3-propanediol, 3.82 g/L 3-hydroxypropionic acid and 6.86 g/L 2,3-butanediol in a 5 L bioreactor using glycerol as the sole carbon source. Overall these results indicate that Klebsiella sp. AA405 is a promising strain for the production of bulk chemicals.
Highlights
Biorefinery has garnered much attention due to depletion of oil reserves and deterioration of environment
When glucose is supplied as carbon source, K. pneumoniae can synthesize a panel of value-added chemicals, including 2,3-butanediol (2,3BDO), acetoin and pyrroloquinoline quinon (PQQ) [4,5]
On the other hand, when glycerol is provided as carbon source, K. pneumoniae turns to generate a range of bulk chemicals, including 1,3-propanediol (1,3-PDO), 3-hydroxypropionic acid (3-HP) and 1-butanol [8,9,10]
Summary
Biorefinery has garnered much attention due to depletion of oil reserves and deterioration of environment. Among recently reported industrial species, Klebsiella pneumoniae is of great attractiveness due to the innate ability to produce chemicals and efficient utilization of glucose and glycerol. When glucose is supplied as carbon source, K. pneumoniae can synthesize a panel of value-added chemicals, including 2,3-butanediol (2,3BDO), acetoin and pyrroloquinoline quinon (PQQ) [4,5]. The titers and productivities can be enhanced by reprogramming metabolic flux because biosynthesis pathways have been well documented [6,7]. On the other hand, when glycerol is provided as carbon source, K. pneumoniae turns to generate a range of bulk chemicals, including 1,3-propanediol (1,3-PDO), 3-hydroxypropionic acid (3-HP) and 1-butanol [8,9,10]. Compared with glucose-utilizing pathways, glycerol-based biosynthesis is more appealing because of inexpensive glycerol, which is the major byproduct of biodiesel industry [12]
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