Abstract
Whey was used as a source of isolation of bacterial strains, symbolized as, IB1, IB2 and IB3, capable of synthesizing β-galactosidase. The microbe labeled as IB1 was tested to be most tolerant against pH and temperature shocks, as well as, heavy metals. Subsequently, IB1 was identified as Bacillus safensis (JUCHE 1) by genetic information. In a later exercise, it was used for β-galactosidase production using whey through fermentative route. The initial concentration of substrate, i.e., lactose in microbial growth medium was varied ranging from of 5-50 g/L. It was found that the classical Monod kinetics and substrate inhibited Monod kinetics are able to describe the microbial growth kinetics at low (5-20 g/L), and high (>20 g/L) concentration ranges of lactose in growth medium respectively. Kinetics of β-galactosidase production followed the Monod incorporated modified Luedeking-Piret model and the Monod incorporated Luedeking-Piret model with substrate inhibition in the low, and high ranges of lactose concentration in growth medium respectively.
Highlights
Whey, the major waste product of dairy industry, is a potential contaminant with a BOD value between 30,000 ppm to 50,000 ppm and COD value between 60,000 ppm to 80,000 ppm
Β-galactosidase may be produced using different microorganisms, bacterial sources are recommended for β-galactosidase production because it provides high yield of enzyme and they are considered as ‘Generally Recognized as Safe’ microorganism [6,7]
5 Conclusions Under the present investigation three bacterial strain IB1, IB2 and IB3 capable of β-galactosidase synthesis have been isolated from casein whey
Summary
The major waste product of dairy industry, is a potential contaminant with a BOD value between 30,000 ppm to 50,000 ppm and COD value between 60,000 ppm to 80,000 ppm. The enzyme β-galactosidase is the key biomolecule used for the treatment of population suffering by hypolactasia, reduction of crystallization of lactose, increase the solubility of milk product, etc. It is an exoglycosidase which hydrolyzes the β-glycosidic bond formed between galactose and its organic moiety. It is expected that the bacterial strains isolated from dairy effluent are more suitable for the production of β-galactosidase using whey This is the reason why few research studies have recently been reported on isolation of β-galactosidase producing bacteria from dairy effluent [8,9,10]. Few research studies have been reported on kinetics of β-galactosidase production through microbial route [11,12,13,14,15]
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