Production mechanism of semicarbazide from protein in Chinese softshell turtles at different drying temperatures based on TMT-tagged quantitative proteomics

Abstract

To investigate the production mechanism of semicarbazide from protein in Chinese softshell turtles at different drying temperatures, the tandem mass tags -tagged protein quantification technique and tandem mass spectrometry were used to screen the differentially abundant proteins of Chinese softshell turtles. Results showed that the protein content in the shells showed a decreasing trend with the increase of drying temperature, and the difference between the protein contents at 70 ℃ and 100 ℃ was significant (p < 0.05). It was found that 13 differentially abundant proteins were significantly correlated with the change in the amount of semicarbazide. As the drying temperature increased, semicarbazide was generated when the target differential abundance proteins were decomposed with the increase of thermal energy. These results provide a basis for more accurate detection of semicarbazide content in crustacean-based aquatic products from the perspective of proteomics.