Abstract
The thermophilic fungus Talaromyces emersonii CBS 814.70 was shown to produce an amylase system during growth on soluble starch. The effect of starch substrate on amylase production during growth of the organism has been examined. During fractionation of the proteins in the extracellular culture filtrate, it has been shown that those filtrates contain at least one form of α-amylase and one form of α-glucosidase. A purified α-amylase has been shown to have a wide pH optimum, ranging between 4.0 and 5.5 with significant quantities of activity remaining at pH 2.5. The enzyme was shown to be thermostable, having a half-life of over 30 min at 80°C. From the data obtained from our studies, it would appear that this enzyme may find use in a number of biotechnological applications.
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