Production, immobilization and application of invertase from new wild strain Cunninghamella echinulata PA3S12MM.

Abstract

The objective of this study was to determine the best conditions to produce invertase by Cunninghamella echinulata PA3S12MM and to immobilize and apply the enzyme. The maximum production was verified in 8days of cultivation at 28°C supplemented with 10gL-1 apple peel, reaching 1054.85Uml-1 . The invertase was purified from the DEAE-Sephadex column. The derivative immobilized in alginate-gelatin-calcium phosphate showed reusability >50% for 19 cycles. The derivative immobilized in glutaraldehyde-chitosan showed greater thermostability and at a different pH. The hydrolysis of 15ml of sucrose 500gL-1 in a fixed bed reactor (total volume of 31ml) produced 24.44µmolmin-1 of glucose and fructose at a residence time of 30min and a conversion factor of 0.5. The new wild strain C. echinulata PA3S12MM presents high invertase production in medium supplemented with an agro-industrial residue and the immobilized enzyme showed high thermal stability and resistance at a different pH. The fungus C. echinulata PA3S12MM is an excellent producer of invertases in Vogel medium supplemented with apple peel. The enzyme is promising for industrial application since it has good performance in reusability and inverted sugar production.