Abstract
The genus Leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides (EPS). These biopolymers possess considerable commercial potential. Because of the wide variety of industrial applications of EPS, this study aimed to produce and characterize the native exopolysaccharide strain Leuconostoc pseudomesenteroides R2, which was isolated from cabbage collected in a semi-arid region of Bahia. We employed the following conditions for the production of EPS: 10.7% sucrose, pH 8.2, without agitation and incubation at 28ºC for 30 hours. The fermentation broth was treated with ethanol and generated two types of polysaccharide substances (EPS I and EPS II). The identification of EPS I and EPS II was conducted using FT-IR, (1)H, (13)C and DEPT-135 NMR spectra. The two substances were identified as linear dextran α polysaccharides (1 → 6) which indicated different characteristics with respect to thermal analysis and density of free packaging, viscosity and time of solubilization. Both dextrans are of low density, possess high thermal stability and exhibited the behavior characteristic of pseudoplastic polymers.
Highlights
Microbial polysaccharides possess rheological pro perties that are conducive to industrial applications and can be produced in large quantities and at highThe exopolysaccharides (EPSs) are composed of secondary metabolites produced when some microorganisms are not in conditions favorable to their proliferation (Mesomo et al 2009)
Most lactic acid bacteria are harmless to human health, and under optimal conditions, many species are capable of producing EPSs in large quantities, providing a suitable option in the use of this polymer in products intended for human consumption (Ricciardi and Clementi 2000, Laws et al 2001)
We used a bacterial strain isolated from cabbage collected in the semi-arid region of Bahia that we identified by a molecular method (16S rRNA) as Leuconostoc pseudomesenteroides
Summary
Microbial polysaccharides possess rheological pro perties that are conducive to industrial applications and can be produced in large quantities and at highThe exopolysaccharides (EPSs) are composed of secondary metabolites produced when some microorganisms are not in conditions favorable to their proliferation (Mesomo et al 2009). Microbial polysaccharides possess rheological pro perties that are conducive to industrial applications and can be produced in large quantities and at high. Research investigating industrial applications is concentrated on the extracellular polysaccharides, which have simpler extraction and purification processes and can be produced in greater quantities (Sutherland 2002). Most lactic acid bacteria are harmless to human health, and under optimal conditions, many species are capable of producing EPSs in large quantities, providing a suitable option in the use of this polymer in products intended for human consumption (Ricciardi and Clementi 2000, Laws et al 2001). Various steps are employed to obtain the EPSs produced in microbial cultures that involve a combination of extraction, purification and quantification techniques (Galindo 1994)
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