Production, Estimation and Protein Network Studies of Bioethanol Fermented Media from Maduca Longifolia Produced by Surface Fermentation using Candida Albicans

Abstract

Candida albicans is a fungus that is used as industrially important species in fermentation productions from past several decades. In the present experimentation, Madhuca longifolia dried flowers with Candida albicans, Saccharomyces cerevisiae, and Pseudomonas aeruginosa respectively are used as cultures and was analyzed for bioethanol production. Bioethanol produced in M. longifolia+yeast with about 40% was more compared with other samples. M. longifolia+ Pseudomonas showed 20% ethanol, M. longifolia+ candida 14% and M. longifolia fermentation, only 5%. There is a good interaction in orange, strawberry, cucumber, apple, cabbage and papaya compared with other samples like grapes, general spinach, red spinach and fenugreek. There is a presence of 24 mg/ml proteins in fermented product of M. longifolia with Candida. The samples have band observation at same distance with standard. Hence the standard marker (Albumin) has 66.5 kD, the fermented product may also have protein with molecular weight of 66.5 kD. The protein interaction studies of albumin with several other proteins of ethanol production showed network with AHSG (alpha-2-HS-glycoprotein), APOA1 (Apolipoprotein A-I), EGF (Epidermal growth factor), GSR (Glutathione reductase), PROS1 (Protein S (alpha)), IGF1 (Insulin-like growth factor 1 (somatomedin C)) etc. All these proteins are involved in body factors in the alcohol production and metabolism. Keywords: Bioethanol production, protein network, Candida albicans, Saccharomyces cerevisiae, Pseudomonas aeruginosa Cite this Article Suprity Saha, Kaladhar DSVGK. Production, Estimation and Protein Network Studies of Bioethanol Fermented Media from Maduca Longifolia Produced by Surface Fermentation using Candida Albicans. Research & Reviews: Journal of Computational Biology. 2018; 7(1): 9–14p.