Production Enhancement of Bacterial Cellulase Cocktail Using Potato Peels Waste Feedstock and Combination of Water Hyacinth Root and Pea Pod Extract as Natural Nutrient Media: Application in Bioconversion of Potato Peels.

Abstract

Solid wastes are the major contributors in global environmental pollution and their management is the need of urgency towards development of sustainable world. In the present work, solid waste of potato peels has been used as feedstock for fermentation of bacterial cellulase production and substrate for enzymatic hydrolysis via this enzymes cocktail. Additionally, liquid extracts of pea pod and root of water hyacinth wastes have been used to complete nutritional requirements and moisture balance in SSF process during the course of enzyme production. At optimum feedstock concentration of 6.0g PPW and 10:40 extract-based moisture ratio of WHR and Ppw, Bacillus sp.produced 15 U/gds FP in 18h, whereas maximum 36 U/gds BGL and 42 U/gds EG have been recorded in 24h of SSF. Temperature 35°C and pH 5.5 were optimum for enzyme production while the produced enzyme was thermally stable upto 30h at 35°C with 100% pH stability upto 14h and 77% relative activity at 34h. The optimized bacterial enzymes have been used for bioconversion of PPW biomass and 26g/L glucose has been recorded at a hydrolytic temperature of 50°C and pH 5.0. The study may have feasible promising scope in cellulosic biorefineries and waste management.