PRODUCTION CORDYLINE FRUTICOSA, L. BY USING TISSUE CULTURE

Abstract

Under in vitro conduction this experiment was carried out at Tissue Culture laboratory at ,Horticulture Research Institute, Agriculture Research Center, during 2009 – 2010 year to substitute chemical hormones with natural compound at establishing stage with studying proliferation ,rooting and acclimatization of Cordyline fruticosa, explants. The results emphasized that using 20% Clorox for 20 min for tips' and 10-20 min, for node with immersion in 70% ethyl alcohol for five seconds gave the best results (100%) survival and free contamination of this explants. Pollen grains collected by hand or bee at 4.00g/l induced significantly increased in callus production, explants development, survival% ,length of shoots ,number of formed leaves but not reached to the level of significance in increasing number of formed shoots for both types of explants tips and one node .One node cutting failed in rooting by addition the two types of pollen grains to cultured medium. Furthermore the use of1.0 mg/l for IBA , NAA or IAA were the best concentrations for callus formation, explants development and survival% .Using MS medium contained 1.0 mg/l NAA and 2.0 mg/l BA or KI significantly increased number and length of shoots, BA was better than KI. The shoots of Cordyline fruticosa, successfully rooted when they cultured in MS medium supplemented with 2.0 mg/l IBA or NAA or IAA. Plantlets after root development formulation of the agricultural media of sand and peat moss at a ratio of 1:3 were effective in increasing survival %,and acclimatization of plant parameters expressed by plant height, shoot thickness and number of leaves. So, it could be recommend to use pollen grains for substitute chemical hormones but it's not commercial. Cultured explants on MS medium contained 1.0mg/l.(NAA or IBA or IAA)and 2.0mg/lBA for producing the best cordyline production. In addition ,using the mixture of sand and peatmoss at a ratio 1-3 for the best acclimatization of Cordyline fruticosa,plant .