Production, characterization and optimization of fibrinolytic protease from Bacillus pseudomycoides strain MA02 isolated from poultry slaughter house soils

Abstract

Abstract Bacterial fibrinolytic enzyme possessed significant application in treatment and prevention of cardiovascular diseases, thus isolation of potential strains producing more fibrinolytic enzyme is of prime importance. In this present study, microorganisms were isolated from soil samples and sediments around poultry slaughter houses and were screened for protease activity. Positive protease producers were further screened for fibrinolytic activity and its efficacy were assessed by fibrinolytic enzyme assay. One strain showed maximum activity of 79.83 FU/mL and the strain was identified as Bacillus pseudomycoides strain MA02. The molecular weight of the protease was estimated to be 35 kDa. Response Surface Methodology (RSM) and Central Composite Rotary Design (CCRD) were used to optimize skimmed milk agar to study the effects of the components in the production of fibrinolytic enzyme by B. pseudomycoides. The highest enzyme activity of 284 FU/mL was obtained at 7.5 g/L of peptone, 45 g/L of sodium chloride and 5 g/L of skimmed milk, which was 3 fold higher than the un-optimized medium.