Abstract
The production, purification and antibacterial effect of chitosan from Mucor rouxii strain DSM-1191 were investigated throughout current study. Chitosan was characterized by infrared spectroscopy, titration and average molecular weight. Furthermore, the antibacterial activity of chitosan produced from Mucor rouxii DSM-1191, against Escherichia coli and Micrococcus leutus was determined. Scanning electron micrographs of treated Micrococcus leutus with fungal chitosan are also presented.
Highlights
Chitosan is a cationic biopolymer consisting of β-(1→4) linked N-acetyl-D-glucosamine prepared from chitin by deacetylation with alkaline solution as shown in (Figure 1).Over the last several years, chitinous polymers, especially chitosan, have received increased attention as one of the promising renewable polymeric materials for their extensive applications in the food industries [1], cosmetology [2,3], biotechnology [4], agriculture [5, 6] in addition to its pharmacology and medicine uses [7,8].Chitosan can be found in the cell wall of certain groups of fungi, zygomycetes
The growth of M. rouxii as well as chitosan production was dependent on the nutrients used and chemico-physical environment of the fermentation medium [17]
In this study the maximum value of the dry cell biomass of M. rouxii DSM-1191 strain under the fermentation condition with YBG culture medium at 28°C, 200 rpm was 24.7 g after 48 h of incubation the amount of the biomass decreased as the amount of nutrients decreased
Summary
Chitosan is a cationic biopolymer consisting of β-(1→4) linked N-acetyl-D-glucosamine prepared from chitin by deacetylation with alkaline solution as shown in (Figure 1).Over the last several years, chitinous polymers, especially chitosan, have received increased attention as one of the promising renewable polymeric materials for their extensive applications in the food industries (preservative, antimicrobial, coating, antioxidant) [1], cosmetology (hair additives, lotions, facial and body creams) [2,3], biotechnology (emulsifier, chelator, flocculent) [4], agriculture (fungicide, films, soil modifier, elicitor) [5, 6] in addition to its pharmacology and medicine uses (fabrics, fibers, artificial organs, drugs, membranes) [7,8].Chitosan can be found in the cell wall of certain groups of fungi, zygomycetes. The object of the current study is to produce chitosan using Mucor rouxii DSM- 1191as a source with an attempt to study physochemical properties of the prepared chitosan as a natural antibacterial agent against a gram-negative bacterium Escherichia coli and a gram–positive Micrococcus lutues strains.
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