Abstract
Optimization of process parameters for phytase production by Enterobacter sp. ACSS led to a 4.6-fold improvement in submerged fermentation, which was enhanced further in fed-batch fermentation. The purified 62kDa monomeric phytase was optimally active at pH 2.5 and 60°C and retained activity over a wide range of temperature (40-80°C) and pH (2.0-6.0) with a half-life of 11.3min at 80°C. The kinetic parameters K m, V max, K cat, and K cat/K m of the pure phytase were 0.21mM, 131.58nmolmg(-1)s(-1), 1.64×10(3)s(-1), and 7.81×10(6)M(-1)s(-1), respectively. The enzyme was fairly stable in the presence of pepsin under physiological conditions. It was stimulated by Ca(+2), Mg(+2) and Mn(+2), but inhibited by Zn(+2), Cu(+2), Fe(+2), Pb(+2), Ba(+2) and surfactants. The enzyme can be applied in dephytinizing animal feeds, and the baking industry.
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