Abstract
Field studies of perennial Nectria canker of northern hardwoods, caused by the ascomycete fungus Nectria galligena, are time-consuming since the disease develops slowly on the stem of trees. In this report, an in vitro bioassay is described for determining and comparing the virulence of different isolates of Nectria galligena by using calli produced from yellow birch buds. The technique facilitates the distinction between highly virulent and less virulent isolates of the pathogen within one week following the inoculation.
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