Abstract
WHEN human peripheral blood leucocytes from two individuals are mixed and cultured, large lymphoblastoid cells appear, the incorporation of thymidine into DNA is increased1,2 and a material which stimulates blastogenesis of allogeneic lymphocytes accumulates in the medium3–5. I wish to describe the production and characteristics of blastogenic factor in mixed cultures of lymphocytes from a pair of twin sisters and from a pair of unrelated individuals (W. and C.) whose cells consistently reacted only slightly in mixed cultures.
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