Abstract
Investigations of polyamine metabolism by microorganisms yielded acetylpolyamine amidohydrolase with a broad substrate specificity and genetic engineering made it possible to produce a large quantity of bacterial N-acetylneuraminate lyase. Acetylpolyamine amidohydrolase and N-acetylneuraminate lyase have been applied respectively to the assay of elevated urinary acetylpolyamine and serum sialic acid concentrations, both of which have proved to be valuable clinical indexes of change in tumor burden.
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