Abstract
The research was focused on the production of lipid based vectors for gene therapy by microfluidic devices, particularly carionic liposomes (CL), and their complexation with plasmidic DNA (pDNA). Two types of microfluidic devices were proposed to achieve the goals, a diffusion based microfluidic device (D-MD) and a caotic advection based microfluidic device (CA-MD). The research showed that the CA-MD has a better productivity of CL while maintaining good properties. In the complexation studies, the D-MD was capable of make the complexes of genetic materials and liposomes (Lipoplexes).
Highlights
Gene therapy is the delivery of a genetic material (DNA or RNA) to a specific cell to achieve a therapeutical response
The incorporation of polyethylene glycol (PEG) has a great impact on the use of carionic liposomes (CL) in gene therapy because it provides the reduction of the size of the vesicle, stabilization of the particles and the better formation of the lipid bilayer due to a steric barrier, give an inert aspect to the particle and accumulate in cancerous tissues by the enhanced permeability and retention effect
The production of CL (EPC, DOTAP and DOPE) was initially carried out using diffusion based microfluidic device (D-MD) and produced samples were analyzed with Dynamic Light Scattering (Zetasizer Nano Malvern)
Summary
Gene therapy is the delivery of a genetic material (DNA or RNA) to a specific cell to achieve a therapeutical response. The immune system servers as a great barrier for the genetic material to surpass and enter the cell. Vectors were proposed with the function to protect and delivery the genes to the targets cells.
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