Abstract
Amylase producing actinobacteria were isolated and characterized from terrestrial environment. There are a limited number of reports investigating the marine environment; hence, in the present study, four marine enzymes were tested for their amylase production ability. On starch agar plates, the Streptomyces rochei strain showed a higher hydrolytic zone (24 mm) than the other isolates. Growth under optimized culture conditions using Plackett-Burman's experimental design led to a 1.7, 9.8, 7.7, and 3.12-fold increase for the isolates S. griseorubens, S. rochei, S. parvus, and Streptomyces sp., respectively, in the specific activity measurement. When applying the Box-Behnken design on S. rochei using the most significant parameters (starch, K2HPO4, pH, and temperature), there was a 12.22-fold increase in the specific activity measurement 7.37 U/mg. The α-amylase was partially purified, and its molecular weight was determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. α-Amylase was particularly active at pH 6 and 65°C. The purified enzyme was most active at 65°C and pH 6, thermal stability of 70°C for 40 min, and salt concentration of 1 M with Km and Vmax of 6.58 mg/ml and 21.93 μmol/ml/min, respectively. The α-amylase was improved by adding Cu+2, Zn+2, and Fe+2 (152.21%, 207.24%, and 111.89%). Increased production of α-amylase enzyme by S. rochei KR108310 leads to production of significant industrial products.
Highlights
Marine habitats are regarded as a potential resource for bioactive metabolites of enormous and unparalleled industrial value
Preliminary screening showed that α-amylase is produced from isolated marine actinomycetes on culture plates
The Streptomyces rochei strain HMM 13 was very active on starch agar plates with iodine as a detector (24 mm) when compared to other isolates (Table 4)
Summary
Marine habitats are regarded as a potential resource for bioactive metabolites of enormous and unparalleled industrial value. About 20,000 natural products, including nine licensed medicines and 12 under clinical trials, have been recorded from marine sources [1]. Extensive marine secondary metabolite varieties have been used for the production of medicinal products. Microorganisms function as a complement to drug development, as they are an excellent source for synthesizing various useful enzymes. Marine microbial enzymes have been found to be rapid, costeffective to produce, and environmentally sound [2]. In extreme circumstances, such as under alkaline and high temperature conditions, most enzymes are more frequently unstable in industrial processes. Researchers are interested in exploring marine microbes that tolerate extreme environmental conditions as a valuable natural resource of new products, including different enzymes [3]
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