Abstract

In vitro experiment was carried out to see the effect of saw dusts of Pinus kesiya, Shorea robusta, and Callicarpa arborea on Trichoderma harzianum, isolate TH-13 mass production, along with its biotization effect on Capsicum chinensis Jacq. “Bhut Jolokia.” Early mycelium initiation (2 days) occurred in S. robusta followed by P. kesiya and C. arborea (3.5 days). The sporulation was observed earlier in S. robusta (100% after 6 days) than P. kesiya (33.4% after 8 days) and C. arborea (16.7% after 9 days) but no sporulation was observed in control. The complete sporulation was also earlier in S. robusta (100% after 10 days) than P. kesiya (33.4% after 15 days) and C. arborea (16.4% after 18 days). Higher conidial yield (86 × 106) was also in S. robusta than P. kesiya (70 × 106) and C. arborea (45 × 106), respectively. The increase in height (60–70 cm), number of leaves (600–650), and yield of chili (120–150 fruits) were also more in inoculated C. chinensis seedlings than control. It is concluded that S. robusta saw dust is the best substrate for mass production of compost fungal activator and can be used in nursery practices for quality stock production of various crops/plantations.

Highlights

  • Compost is a mixture of decayed organic material decomposed by microorganisms in a warm, moist, and aerobic environment that release nutrients into readily available forms for plant use

  • Pers. are important microbes that help in decomposition of organic material and are known as “compost fungal activator” (CFA)

  • Fungal species Trichoderma harzianum was isolated from the soil samples by using serial soil dilution method [13] on potato dextrose agar (PDA) medium

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Summary

Introduction

Compost is a mixture of decayed organic material decomposed by microorganisms in a warm, moist, and aerobic environment that release nutrients into readily available forms for plant use. A study is carried out to analyze the different substrates (saw dusts) suitability for mass production of CFA along with its effect on growth and development of Capsicum chinensis Jacq. The inoculum containing medium was cut into small discs and was put in flasks containing wheat bran and different saw-dust medium in the ratio (3 : 1 : 4 w/w) for mass production of Trichoderma harzianum.

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