Abstract
The soil bacterium Tsukamurella spec. DSM 44370 produces a mixture of oligosaccharide lipids when cultivated on sunflower oil. In contrast cultivation with calendula oil as carbon source afforded a different product composition with overproduction of 2,3-di- O-acyl-β- d-glucopyranosyl-(1–2)-β- d-galactopyranosyl-(1–6)-4,6-di- O-acyl-α- d-glucopyranosyl-(1–1)-α- d-glucopyranose (GL 3) that amounted to 60% of the whole product. GL 3 and its parent tetrahexose backbone G 3 were then modified enzymatically with the lipase Novozyme 435 from Candida antarctica by addition of one and two oleic acid molecules to GL 3 and four molecules to G 3. The new glycolipids were shown to exhibit interesting surface activities compared to commercially available products, decreasing the surface tension of water to 23 mN/m. In addition these products showed novel biological activities through the inhibition of the activation of Epstein-Barr virus early antigen.
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