Abstract
Techniques are described for the production of anti-idiotypic B cells by immunization with idiotypic antibodies complexed to rheumatoid factor. Screening for anti-idiotypic antibody production and immunoaffinity isolation of the B cells, actively producing the anti-idiotype antibody, by idiotypic antibody-coated magnetic beads is described. This methodology can be used to isolate anti-idiotypic B cells for immune regulation studies or to provide fusion partners for the production of monoclonal anti-idiotypic antibodies.
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Schedule a callMore From: Journal of tissue culture methods : Tissue Culture Association manual of cell, tissue, and organ culture procedures
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