Production and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020.

Elena V Liyaskina,Philip V Toukach,Valentina V Rusyaeva,Victor V Revin,Alexey Fomenkov,Alevtina A Kitykina,Saulius Vainauskas,Nadezhda A Rakova,Richard J Roberts,Dharam Singh

doi:10.1371/journal.pone.0253482

Abstract

Paenibacillus spp. exopolysaccharides (EPSs) have become a growing interest recently as a source of biomaterials. In this study, we characterized Paenibacillus polymyxa 2020 strain, which produces a large quantity of EPS (up to 68 g/L),and was isolated from wasp honeycombs. Here we report its complete genome sequence and full methylome analysis detected by Pacific Biosciences SMRT sequencing. Moreover, bioinformatic analysis identified a putative levan synthetic operon. SacC and sacB genes have been cloned and their products identified as glycoside hydrolase and levansucrase respectively. The Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra demonstrated that the EPS is a linear β-(2→6)-linked fructan (levan). The structure and properties of levan polymer produced from sucrose and molasses were analyzed by FT-IR, NMR, scanning electron microscopy (SEM), high performance size exclusion chromatography (HPSEC), thermogravimetric analysis (TGA), cytotoxicity tests and showed low toxicity and high biocompatibility. Thus, P. polymyxa 2020 could be an exceptional cost-effective source for the industrial production of levan-type EPSs and to obtain functional biomaterials based on it for a broad range of applications, including bioengineering.

Highlights

Interest in the biosynthesis and production of different EPSs has increased considerably in recent years, because they are potential candidates and precursors for many commercial applications in different industries, including but not limited to food, petroleum, pharmaceuticals and biomedicine [1]
In comparison to the media with sucrose, in the molasses medium, the maximum production of polysaccharide was achieved over a shorter time interval (48–72 h)
This is the maximum levan yield on sucrose-containing media in comparison with the literature data known for P. polymyxa

Summary

Introduction

Interest in the biosynthesis and production of different EPSs has increased considerably in recent years, because they are potential candidates and precursors for many commercial applications in different industries, including but not limited to food, petroleum, pharmaceuticals and biomedicine [1]. The original sequence reads have been deposited at NCBI under SRA SRR11271650 and SRR11271651. The original sequence reads have been deposited at NCBI under SRA: SRR11236808; SRR11236809; SRR11236810; SRR11236811.

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