Abstract
A transgenic line of the sugarcane cultivar Q124 with significantly enhanced resistance to Fiji disease was produced by microprojectile-mediated transformation with a transgene encoding a translatable version of Fiji disease virus (FDV) segment 9 ORF 1 under the control of the maize polyubiquitin promoter. Sixty-four transgenic lines were tested in glasshouse trials by caging the plants with viruliferous Perkinsiella saccharicida planthoppers. After 2 weeks, the planthoppers were removed and the plants monitored for symptoms. One transgenic line showed significantly enhanced resistance to Fiji disease compared with the Q124 parent and other lines showed varying levels of resistance. The molecular phenotypes of the transgenic plants at both the DNA and RNA levels were not entirely consistent with a resistance mechanism based on post-transcriptional gene silencing but were consistent with reports from other sugarcane-virus resistance systems. This is the first report of transgenic sugarcane containing an FDV-derived synthetic resistance gene showing resistance to FDV, although the mechanism of resistance has not yet been elucidated.
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