Abstract
We have produced a series of monospecific antibodies in five rabbits by immunization with a peptide corresponding to the preprogastrin sequence 76-88. Both antibody titres (0.2 to 3.0 x 10(6)), indexes of heterogeneity (approximately 1.0), and binding affinities (Koeff approximately 0.2 to 3.7 x 10(12) 1 x mol-1) were very high. The specificity for the N-terminus of the progastrin fragment was unique because even a conservative amino acid substitution five positions from the centre of binding diminished the binding by 90% for all antibodies. We conclude that these monospecific antibodies are well suited for development of a processing-independent analysis of progastrin and its products.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Scandinavian Journal of Clinical and Laboratory Investigation
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
