Production and evaluation of doubled haploid lines of barley via detached-tiller culture method

Abstract

This research was conducted to compare the classic (C) and detached-tiller methods (a: sterile by clip and scissors D1; b: sterile by hot water D2) for producing haploids in barley. The parental materials used in this research were F1 four genotypes ofHordeum vulgare: B1: Reihane × Legia; B2: Reihan × Igri; B3: Kavir × Igri; B4: Kavir × Legia, and the Hordeum bulbosum was PB1 genotype (Plant Breeding Institute). The traits such as seed set percentage, embryo development, haploid seedling development and produced doubled haploid lines were analyzed statically using c2test. The comparison was made among arable barley B1, B2, B3, B4 crossing wild barley of H. bulbosum. In C approach, there was no significant difference concerning the percent of forming seed, embryo and haploid production. While in D1 approach, there was a significant difference in embryo forming percent but no significant difference was observed for haploid plant and seed set percent. Moreover, in D2approach, there was a significant difference for seed set percent. However, for haploid production and embryo development percent, the approach D1 in regards with the percent of forming embryo and production of haploids was superior to the other two approaches, C and D2. Key words: Detached-tiller culture, haploid, doubled haploid, Hordeum bulbosum, Hordeum vulgare.