Abstract

With 1 figure and 1 table AbstractThe alloplasmic line of radish carrying Brassica maurorum cytoplasm was obtained from four backcrosses (BC) of the amphidiploid hybrid (B. maurorum × Raphanus sativus) to radish. Eight BC1 plants (2n = 26) and one BC2 plant (2n = 22) were generated only by using embryo rescue, and two BC3 plants (2n = 19, 20) were obtained by repeat pollinations after unbagged bud pollination and without embryo rescue. After successive backcrossings with 10 radish cultivars to BC6 generation, the resulting alloplasmic line of radish (2n = 18) showed stable expression of male sterility without any recognizable changes in vegetative growth and female fertility. This cytoplasmic male sterility (CMS) line of radish was dissimilar to the other existing CMS lines with respect to cytogenetic and molecular characteristics. The novel CMS line produced in the present investigation may be a versatile germplasm for F1 hybrid seed production of radish.

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