Production and Characterization of Poly (Lactic Acid)/Nanostructured Carboapatite for 3D Printing of Bioactive Scaffolds for Bone Tissue Engineering.

Abstract

Biocompatible scaffolds are porous matrices that are bone substitutes with great potential in tissue regeneration. For this, these scaffolds need to have bioactivity and biodegradability. From this perspective, 3D printing presents itself as one of the techniques with the greatest potential for scaffold manufacturing with porosity and established structure, based on 3D digital modeling. Thus, the objective of the present work was to produce 3D scaffolds from the poly (lactic acid) (PLA) and the nanostructured hydroxyapatite doped with carbonate ions (CHA). For this purpose, filaments were produced via fusion for the fused-filament 3D printing and used to produce scaffolds with 50% porosity in the cubic shape and 0/90°configuration. The dispersive energy spectroscopy and Fourier transform infrared spectroscopy (FTIR) analysis demonstrated the presence of CHA in the polymeric matrix, confirming the presence and incorporation into the composite. The thermogravimetric analysis made it possible to determine that the filler concentration incorporated in the matrix was very similar to the proposed percentage, indicating that there were no major losses in the process of obtaining the filaments. It can be assumed that the influence of CHA as a filler presents better mechanical properties up to a certain amount. The biological results point to a great potential for the application of PLA/CHA scaffolds in bone tissue engineering with effective cell adhesion, proliferation, biocompatibility, and no cytotoxicity effects.