Production and characterization of monoclonal antibodies against mink leukocytes

Abstract

Three monoclonal antibodies (mAbs) were generated against mink leukocytes. One antibody reacted with all T lymphocytes, one with all monocytes and one had platelet reactivity. Under reducing conditions, the T lymphocyte reactive antibody immunoprecipitated 18 kDa, 23 kDa, 25 kDa and 32–40 kDa polypeptides and the platelet reactive antibody 17 kDa, 22 kDa plus two high molecular weight (> 100 kDa) polypeptides. Immunohistological studies of the mAbs were performed in order to localize the cellular distribution of the detected antigens in various organs. The T lymphocyte reactive antibody detected an antigen, which was widely distributed in the T cell area of lymph nodes and spleen and in the thymic medulla. We conclude that this antibody is an anti-CD3 mAb and suggest that the platelet reactive antibody reacts to the CD41 CD61 integrin molecule. In addition to our own mAbs, more than 100 mAbs against leukocytes of human and various animal species have been analysed for cross-reactivity to mink leukocytes. We found eight to cross-react with mink. Of particular importance was an anticanine CD11a mAb, an antihuman CD79a mAb and an antihuman bcl-2 mAb.