Abstract
The efficient cellulolytic microorganism was isolated from soil samples collected from Shivaji University campus. Biochemical test and phylogenetic analysis of isolated culture identified as Klebsiella sp. PRW-1. The isolated culture could utilize pure cellulosic substrates (carboxymethylcellulose (CMC) and avicel) and different agricultural wastes like sugarcane bagasse, sugarcane barbojo, sorghum husks, grass powder, corn straw and paddy straw by producing a large amount of endoglucanase, exoglucanase, β-glucosidase, filter paperase (FPU), xylanase and glucoamylase. The reducing sugar production was found higher in the presence of grass powder and sugarcane barbojo. Effects of different physic-chemical parameters to achieve maximum cellulolytic enzymes production were systematically investigated. The effects of supplementation of different metals additives as well as the stability in the presence of higher temperature, pH and commercial detergents, on cellulolytic enzymes were also studied. The foregoing result increases the applicability of the strain for the utilization and bioconversion of lignocellulosic biomass that could be used for bioenergy production.
Highlights
The beginning of the industrial revolution and the consequent tremendous usage of fossil fuels showed negative impacts on the environment mostly due to the emission of greenhouse gases (CO2, CH4 and CO) resulting in global warming and pollution (Saratale et al, 2008)
PRW-1 strain showed its ability to grow on different cellulosic substrates by producing extracellular cellulolytic and hemicellulolytic enzymes under static submerged condition
Enhancement in the reducing sugar production was observed at the 7th day of incubation using grass powder as substrate
Summary
The beginning of the industrial revolution and the consequent tremendous usage of fossil fuels showed negative impacts on the environment mostly due to the emission of greenhouse gases (CO2, CH4 and CO) resulting in global warming and pollution (Saratale et al, 2008). Large efforts are being conducted worldwide for the development of technologies which could generate clean, sustainable energy sources lignocellulosic biomass to biofuels, to substitute fossil fuels (Ragauskas et al, 2006; Levin et al, 2006). Biofuels produced from common agricultural wastes biomass, represent CO2 cycle, ecofriendly, cost competitive with fossil fuels, biodegradable and it contributes to the sustainability (Saratale and Oh, 2012). The produced biofuels becomes important and Received 18 January 2013; Revised 02 May 2013; Accepted 21 May 2013.
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