Production and Characterization of Antifungal Compounds Produced by Lactobacillus plantarum IMAU10014

Haikuan Wang,Wei Qi,Heping Zhang,Jiaming Wang,Yanhua Yan,Mark R Liles

doi:10.1371/journal.pone.0029452

Abstract

Lactobacillus plantarum IMAU10014 was isolated from koumiss that produces a broad spectrum of antifungal compounds, all of which were active against plant pathogenic fungi in an agar plate assay. Two major antifungal compounds were extracted from the cell-free supernatant broth of L. plantarum IMAU10014. 3-phenyllactic acid and Benzeneacetic acid, 2-propenyl ester were carried out by HPLC, LC-MS, GC-MS, NMR analysis. It is the first report that lactic acid bacteria produce antifungal Benzeneacetic acid, 2-propenyl ester. Of these, the antifungal products also have a broad spectrum of antifungal activity, namely against Botrytis cinerea, Glomerella cingulate, Phytophthora drechsleri Tucker, Penicillium citrinum, Penicillium digitatum and Fusarium oxysporum, which was identified by the overlay and well-diffusion assay. F. oxysporum, P. citrinum and P. drechsleri Tucker were the most sensitive among molds.

Highlights

Fungal pathogens cause devastating losses of crops and postharvest fruits throughout the world [1]
Microbial strains and culture conditions The Lactobacillus plantarum culture used in this study was incubated by using the method as described by Wang et al [16]
One loopful of the stock culture of L. plantarum IMAU10014 was streaked onto Man Rogosa Sharpe broth (MRS, Fluka) agar plate and incubated at 37uC for 48,72 h

Summary

Introduction

Fungal pathogens cause devastating losses of crops and postharvest fruits throughout the world [1]. Many artificial chemical fungicides have been used to prevent and kill fungi in various environments. Because of their huge populations and high frequency of mutation, a large amount of pathogenic fungi may acquire resistance to frequently used fungicides. To reduce the risk of crop disease and enhance the safety of food and the environment, new, safer fungicides should be discovered and developed [3]. Using microorganisms to prevent fungal pollution has been sparking and gaining interest during the recent years due to consumers’ demand for reducing potential negative effects chemical fungicides may have on the environment [4]

Methods

Results

Conclusion