Abstract
In animal farming, improvement of growth performance through successful nutrient uptake and digestibility is critical. Phytases hydrolyze the anti-nutritive phytic acid present in grains to lesser derivatives and release the phosphorus trapped therein. Twenty-eight fungi obtained from poultry droppings, cereal-rich soils and garden soils were screened for phosphate solubilization and phytase production using Pikovskaya agar and phytase screening medium. Out of the fungi, 61% were positive for phytase producing abilities and the most proficient, isolate GS1S6 with a solubilization index of 121%, was identified as Aspergillus niger GS1S6. Optimum phytase production was achieved at a fermentation period of 120 h, pH 5.5, glucose, (NH4)2SO4 using 72 hour old fungal inoculum. Mineral supplementation of natural agricultural substrates enhanced phytase production (1000U/L) the most in milled sesame seed medium. The ~31-38 KDa partially purified and characterized enzyme demonstrated optimum activities at 55oC and pH 6.0, while cationic inclusions reduced phytase activities. The Km and Vmax were low (1.308 mM and 0.077 mM/mL/min) from Linear-weaver plot with increasing substrate concentration implying that A. niger GS1S6 phytase may efficiently mineralize phytic acid and therefore hold great prospect for its commercialization.
Highlights
Phytic acid is a major form of phosphorus storage in plants and a constituent of all plant seeds with about 1-5% occurring in many cereals and oilseeds
Screening for phytase and effect of cultivation conditions on phytase production Twenty-eight fungi with phytase producing abilities were obtained in this work and the fungal count in the samples studied varied between 105 and 106 total fungal units (TFU)/g sample (Table 1)
Isolate GS1S6 was selected for phytase production studies
Summary
Phytic acid is a major form of phosphorus storage in plants and a constituent of all plant seeds with about 1-5% occurring in many cereals and oilseeds. They account for 60-90% of the total phosphorus in seeds (Afinah et al 2010; Kalsi et al 2016). Phytase supplementation in feed maximizes nutrients uptake in the animal minimizing the phatate released into environment through the undigested feed within faecal matter (Robinson et al 2002; Vashishth et al 2017). In addition we determined the production and enzyme characteristics of a fungal phytase produced in a submerged fermentation medium containing different underutilized agro-based ‘waste’ materials as fermentation substrates either devoid of, or supplemented with essential nutrients
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