Abstract
A polyclonal antibody to peptide containing 15 amino acids and corresponding to region - D of human estrogen receptors (hERD) was obtained in mice by immunization with the coupler of peptide and KLH. Using this antiserum, the ER status of paraffin - embeded sections of 95 human breast carcinomas were studied. The corresponding rate for determination of ER status between immunohistochemical staining (IHC) and dextran coated charcoal (DOC) assay was 89.5%. The concordance for semiquantitative grades was 69.3%. In addition, insitu hybridization (ISH) of 15 frozen sections of same sample using digoxigenin labeled dUTP to identify the expression of ER mRNA for confirming the 1HC also be used. This technique revealed more specific, sensitive and convenient than DOC. The results of ISH were fully consistent with IHC (100%). Above results show that the mouse antiserum to hERD obtained in this study is specific and sensitive for IHC assay of ER and IHC is a valuable adjunct and/or alternative to the biochemical method for determination of the ER status of breast cancer.
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