Abstract
Cladosporium cladosporiodes is a novel strain isolated and evaluated for the production of laccase in submerged fermentation. Objective of present work was to optimize the laccase production and assessment of delignification in wheat bran by laccase of Cladosporium cladosporiodes in submerged fermentation. The significant laccase production was observed in Byred’s medium when grown on wheat bran at static condition. The optimum conditions i.e. pH 5.6, temperature 40°C, wheat bran 4g, CuSO4 concentration 0.1mM were required for laccase production in submerged fermentation and there was no any role of tested six aromatic inducers on laccase production. The extracellular laccase enzyme of Cladosporium cladosporiodes produced from lignocellulosic material fermentation involved in delignification of wheat bran, biotransformation of lignin model compounds and decolourization eight different azo, metal complex and triphenyl methane dyes.
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