Production and analysis of multiply charged negative ions by liquid atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry.

Oliver J Hale,Pavel Ryumin,Michael Morris,Jeffery M Brown,Rainer Cramer

doi:10.1002/rcm.8246

Oliver J Hale, Pavel Ryumin + Show 3 more

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https://doi.org/10.1002/rcm.8246

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Abstract

RationaleLiquid atmospheric pressure matrix‐assisted laser desorption/ionisation (AP‐MALDI) has been shown to enable the production of electrospray ionisation (ESI)‐like multiply charged analyte ions with little sample consumption and long‐lasting, robust ion yield for sensitive analysis by mass spectrometry (MS). Previous reports have focused on positive ion production. Here, we report an initial optimisation of liquid AP‐MALDI for ESI‐like negative ion production and its application to the analysis of peptides/proteins, DNA and lipids.MethodsThe instrumentation employed for this study is identical to that of earlier liquid AP‐MALDI MS studies for positive analyte ion production with a simple non‐commercial AP ion source that is attached to a Waters Synapt G2‐Si mass spectrometer and incorporates a heated ion transfer tube. The preparation of liquid MALDI matrices is similar to positive ion mode analysis but has been adjusted for negative ion mode by changing the chromophore to 3‐aminoquinoline and 9‐aminoacridine for further improvements.ResultsFor DNA, liquid AP‐MALDI MS analysis benefited from switching to 9‐aminoacridine‐based MALDI samples and the negative ion mode, increasing the number of charges by up to a factor of 2 and the analyte ion signal intensities by more than 10‐fold compared with the positive ion mode. The limit of detection was recorded at around 10 fmol for ATGCAT. For lipids, negative ion mode analysis provided a fully orthogonal set of detected lipids.ConclusionsNegative ion mode is a sensitive alternative to positive ion mode in liquid AP‐MALDI MS analysis. In particular, the analysis of lipids and DNA benefited from the complementarity of the detected lipid species and the vastly greater DNA ion signal intensities in negative ion mode.

Highlights

Substantial advances have recently been made with respect to the production of electrospray ionisation (ESI)‐like multiply charged macromolecules such as peptides and proteins using non‐ESI techniques,[1,2] including laser‐based desorption techniques.[3,4] With regard to the latter techniques, most of these, if not all, have been achieved and reported for the analysis of positive analyte ions
The instrumentation employed for this study is identical to that of earlier liquid AP‐MALDI mass spectrometry (MS) studies for positive analyte ion production with a simple non‐commercial AP ion source that is attached to a Waters Synapt G2‐Si mass spectrometer and incorporates a heated ion transfer tube
In the first set of experiments, two established liquid MALDI sample preparations from earlier positive ion mode studies were used as well as two preparations that were slightly different by swapping the matrix chromophores with compounds which promised to be more suitable for negative ion mode analysis

Summary

Introduction

Substantial advances have recently been made with respect to the production of electrospray ionisation (ESI)‐like multiply charged (bio) macromolecules such as peptides and proteins using non‐ESI techniques,[1,2] including laser‐based desorption techniques.[3,4] With regard to the latter techniques, most of these, if not all, have been achieved and reported for the analysis of positive analyte ions. Results: For DNA, liquid AP‐MALDI MS analysis benefited from switching to 9‐aminoacridine‐based MALDI samples and the negative ion mode, increasing the number of charges by up to a factor of 2 and the analyte ion signal intensities by more than 10‐fold compared with the positive ion mode.

Results

Conclusion