Abstract
In this research,a new expression vector of pCAMBIA1301-35S promoter-igf-1-nos,which included the gene of hIGF-1,was constructed.The gene is promoted by CaMV 35S promoter which can be expressed in tobacco in high performance.The expression vector of IGF-1 cDNA introduced by 35S promoter was transformed into LBA4404(Agrobacterium tumefacients),and then infected tobacco through leaf-disc infection.17 transgenic tobacco plants were gained after hygromycin selection,GUS test and PCR test of DNA.It shows IGF-1 gene can be transcribed into normal mRNA in transgenic tobacco by RT-PCR test.In this experiment,the expression of IGF-1 in tobacco lays the foundation for producing IGF-1 in tobacco and other dicotyledon bio-reactors as well.
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