Abstract
Enzymatic synthesis of structured lipids by transesterification of soybean oil with palm kernel oil was evaluated in a packed-bed reactor with a capacity for 500g of enzyme loading. Lipozyme RM-IM was used as catalyst. Substrate blends were passed through the enzyme bed at different flow rates. Transesterification reached a level of 19.6 %, with a maximum calculated productivity of 2344 kg of transesterified oil/kg of immobilized enzyme, a flow rate of 9,36 kg oil/kg enzyme/h. The triacylglycerols formed in major proportion were C40:2, C42:2, C42:3, C44:2, C44:3, C50:3 and C50:4. Stereoespecific analysis of the fat before and after transesterification shows a slight migration of acyl groups. The products obtained by this technology can be applied in the formulation of lipid emulsions for enteral and parenteral nutrition and the food industry.
Highlights
Enzymatic synthesis of structured lipids by transesterification of soybean oil with palm kernel oil was evaluated in a packed-bed reactor with a capacity for 500g of enzyme loading
Las enzimas inmovilizadas permiten trabajar en reactores continuos tipo lecho empacado (PBR), con una caída de presión mínima a altas velocidades de flujo, además de lograr la reutilización eficiente del sistema biocatalítico (Corredor y Caicedo, 2005)
El análisis de la distribución espacial de los ácidos grasos en los triacilgliceroles se realizó siguiendo la metodología descrita por Dourtoglou (2001), en la cual se utiliza una lipasa 1,3-específica (Lipozyme IM20) en butanol para transformar los ácidos grasos ubicados en las posiciones sn-1,3 de los triacilgliceroles en sus correspondientes butilésteres y cuantificarlos posteriormente por cromatografía de gases
Summary
Production of structured lipids by enzymatic transesterification of soybean oil and palm kernel oil in a packed-bed reactor. Enzymatic synthesis of structured lipids by transesterification of soybean oil with palm kernel oil was evaluated in a packed-bed reactor with a capacity for 500g of enzyme loading. Substrate blends were passed through the enzyme bed at different flow rates. Transesterification reached a level of 19.6 %, with a maximum calculated productivity of 2344 kg of transesterified oil/kg of immobilized enzyme, a flow rate of 9,36 kg oil/kg enzyme/h. The products obtained by this technology can be applied in the formulation of lipid emulsions for enteral and parenteral nutrition and the food industry. KEY-WORDS: Lipase – Lipozyme – Packed-bed reactor – RM-IM – Structured lipids
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