Procollagen-Like Protein as a Molecular Target in the Treatment of Primary Brain Tumor.

Medhi Wangpaichitr,Howard Landy,Jenny Xu,Chun Jing Wu,Niramol Savaraj,Lynn G Feun,Rong Xu

doi:10.1100/tsw.2002.59

Medhi Wangpaichitr, Howard Landy + Show 5 more

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https://doi.org/10.1100/tsw.2002.59

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Abstract

METHODS. cDNA library was obtained in one glioma cell line which highly expressed this protein and screening was performed using VDR monoclonal antibody. This partial cDNA was then subcloned into pcDNA3.1 and sequencing was performed. This partial cDNA sequence also was used as a probe to identify mRNA, which encodes for this putative gene. To characterize this 220-Kd protein, we have used five cell lines as follows: Glioma 1, U118, SW1783, which possess variable amount of 220-Kd protein, and U-373 and T-98G which lack this 220-Kd protein.

Highlights

We have previously reported that the presence of a unique 220-Kd protein, which is recognized by vitamin D receptor (VDR) monoclonal antibody, correlated with vitamin D3 (VD3) sensitivity in primary brain tumor[1]
Via BLAST search through GENBANK, this cDNA fragment was found to be identical to nucleotide 2840-5118 of procollagen alpha 1 type 1
Our results indicate that the immunoprecipitant of cell lysate with VDR antibody reacts with the antibody against the Cterminal peptide of procollagen type 1

Summary

Introduction

We have previously reported that the presence of a unique 220-Kd protein, which is recognized by vitamin D receptor (VDR) monoclonal antibody, correlated with vitamin D3 (VD3) sensitivity in primary brain tumor[1]. CDNA library was obtained in one glioma cell line which highly expressed this protein and screening was performed using VDR monoclonal antibody. This partial cDNA sequence was used as a probe to identify mRNA, which encodes for this putative gene. Via BLAST search through GENBANK, this cDNA fragment was found to be identical to nucleotide 2840-5118 of procollagen alpha 1 type 1.

Results

Conclusion