Prochloraz alone or in combination with nano-CuO promotes the conjugative transfer of antibiotic resistance genes between Escherichia coli in pure water

Abstract

Conjugative plasmid transfer is a major contributor to the spread of antibiotic resistance genes (ARGs). However, the role of conventional fungicides on conjugative plasmid transfer has been neglected. Based on the condition that the increasing use of the combination of nano- and conventional fungicides will lead to combined contamination, the effects of a conventional fungicide prochloraz alone or in combination with nano-CuO on the conjugation of plasmid RP4 between Escherichia coli in phosphate-buffered saline were investigated in this study. The results demonstrated that 50 µg/L prochloraz alone significantly increased the conjugative transfer by 1.82 folds. The combination of 100 µg/L nano-CuO and prochloraz at 5, 50, and 500 µg/L significantly increased the conjugation by 2.56, 3.61, and 2.13 folds, respectively. The promotion of conjugative transfer of ARGs mediated by fungicides is mainly attributed to (i) the increased cell membrane permeability, (ii) the increased cell adhesion via enhancing the synthesis of polysaccharides in extracellular polymeric substances, and (iii) the up-regulation of the genes relevant to conjugation, oxidative stress, SOS response, outer membrane, polysaccharide export, intercellular adhesion, and ATP synthesis. Our findings provide evidence for the contribution of fungicides to ARGs transfer, which is significant to control the risk of ARGs dissemination.