Abstract
Cellulase has myriad applications in various sectors like pharmaceuticals, textile, detergents, animal feed and bioethanol production, etc. The current study focuses on the isolation, screening and optimization of fungal strain through one factor at a time technique for enhanced cellulase production. In current study sixteen different fungal cultures were isolated and the culture which quantitatively exhibits higher titers of cellulase activity was identified both morphologically and molecularly by 18S rDNA and designated as Aspergillus niger ABT11. Different parameters like fermentation medium, volume, temperature, pH and nutritional components were optimized. The highest CMCase and FPase activities was achieved in 100ml of M5 medium in the presence of 1% lactose and sodium nitrate at 30 oC, pH5 after 72 hours. The result revealed A. niger can be a potential candidate for scale up studies.
Highlights
Cellulose is an insoluble crystalline polysaccharide of repeating units of glucose that is linked through β-1, 4-glucosidic bonds
The current study focuses on the isolation, screening and optimization of fungal strain through one factor at a time technique for enhanced cellulase production
The highest CMCase and FPase activities was achieved in 100ml of M5 medium in the presence of 1% lactose and sodium nitrate at 30 oC, pH5 after 72 hours
Summary
Cellulose is an insoluble crystalline polysaccharide of repeating units of glucose that is linked through β-1, 4-glucosidic bonds. Filamentous fungi are preferred over bacteria because of their ability to penetrate deep in cellulosic substrates by using their hyphal extensions and showing their enzyme systems in confined cavities within the cellulosic particles as compared to bacteria that cannot penetrate deep in to the substrate (Lynd et al 2002). In addition to this fungal cellulases has the capability to act on both amorphous and crystalline structures of cellulose in contrast to bacteria (Damisa et al 2011). Most commonly used fungal species for the production of cellulases are Aspergillus niger, Penicillium dupontii, Fusarium oxysporum and Trichoderma viride etc. (Hussain et al 2012)
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