Process Optimization and Characterizations of Enzyme-Extracted and Acid-Extracted Collagen from Scale of Bighead Carp

Abstract

Collagen is an important biomedical material. Enzyme (papain) extracted collagen (EEC) and acid (citric acid) extracted collagen (AEC) were isolated from the scale of bighead carp. The process conditions of EEC and AEC were optimized, by orthogonal test method , to be: liquid/solid ratio 20 mL/g, extraction time 48 min, papain concentration 9.52×10-5 mol/L, temperature 28°C for EEC and liquid/solid ratio 20 mL/g, extraction time 36 min, citric acid concentration 0.5 mol/L, temperature 28°C for AEC.Under the optimal conditions the maximum extraction rates were 31.20% for EEC and 9.22% for AEC, respectively. Both EEC and AEC were characterized basically as type I collagen by UV spectra, infrared spectra and amino acid compositions.