Abstract
Succinic acid (SA) is a top platform chemical obtainable from biomass. The current study evaluated the potential of Actinobacillus succinogenes for SA production using xylose-rich hemicellulosic fractions of two important lignocellulosic feedstocks, olive pits (OP) and sugarcane bagasse (SCB) and the results were compared with pure xylose. Initial experiments were conducted in shake flask followed by batch and fed-batch cultivation in bioreactor. Further separation of SA from the fermented broth was carried out by adapting direct crystallisation method. During fed-batch culture, maximum SA titers of 36.7, 33.6, and 28.7 g/L was achieved on pure xylose, OP and SCB hydrolysates, respectively, with same conversion yield of 0.27 g/g. The recovery yield of SA accumulated on pure xylose, OP and SCB hydrolysates was 79.1, 76.5, and 75.2%, respectively. The results obtained are of substantial value and pave the way for development of sustainable SA biomanufacturing in an integrated biorefinery.
Highlights
Even today, the major fraction of fuels and chemicals are derived from the non-renewable fossil fuels
Samples were withdrawn at regular intervals during the shake flask and bioreactor experiments to analyse for xylose, Succinic acid (SA), ethanol, lactic acid (LA), acetic acid (AA), and formic acid (FA) concentrations using high performance liquid chromatography (HPLC) system
Due to lack of commercially viable microorganisms to utilise xylose, it is overlooked or considered as inferior fermentable sugar for decades which limits the economics of Lignocellulosic biomass (LCB)-based biorefineries (Prabhu et al, 2020; Narisetty et al, 2021)
Summary
The major fraction of fuels and chemicals are derived from the non-renewable fossil fuels. The waste streams rich in fermentable carbon such as agricultural residues, industrial effluents, food and bakery wastes have become valuable resources in this era of circular bio/economy. The concept focuses on recycle, reuse and manufacture with cascading use of biologicals resources from various waste and side streams in a systematic manner, alleviating environ mental concern and resulting in a low carbon economy (Zero Waste Scotland, 2017; Leong et al, 2021). Majority of the work in literature have made use of cellulosic sugars for fermentative production of fuels and chemicals with limited research on hemicellulosic fraction rich in xylose (~90%). In the last two decades, various native and genetically engineered pentose assimilatory micro organisms have been identified and constructed that could valorise the fermentable sugars present in hemicellulosic fraction into an array of chemical products. The efficient conversion of pentose sugars will be essential for augmenting the economic viability and profitability of the LCB-based biorefineries (Kwak et al, 2019; Prabhu et al, 2020; Nar isetty et al, 2021)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
