Process intensification of fluidized bed dye-ligand adsorption of G3PDH from unclarified disrupted yeast: A case study of the performance of a high-density steel–agarose pellicular adsorbent

Abstract

The development of a process intensified primary capture step for the direct selective recovery of intracellular proteins from very dense particulate-containing yeast extract has been explored. The purification of glyceraldehyde 3-phosphate dehydrogenase from bakers’ yeast was chosen as a potential demonstration of this approach. A high throughput (50%, w/v, yeast extracts at a superficial linear velocity of 450 cm h −1) was achieved by adoption of a high-density adsorbent (UpFront steel–agarose; ρ = 2.65 g ml −1) derivatized with selective ligand chemistries (Cibacron Blue 3GA). This should ultimately minimize adsorption time and maximize process efficiency of fluidized bed adsorption.