Abstract
In the present study, the effect of Iron Nanoparticles (Fe NPs), Nickel Nanoparticles (Ni NPs) and co-additions of Iron and Nickel nanoparticles (CNPs) for the production of biohydrogen from the effluent collected brewery processing was investigated. Free and co-culture (immobilized) of EtBr mutated Rhodobacter M 19 and Enterobacter aerogenes wereused for biohydrogen production. The biohydrogen productions is found to be enhanced by using nanoparticles as active catalyst. The results showed the maximum biohydrogen production of 96.21% by using combined nanoparticles (CNPs) with immobilized EtBr mutated co-culture. The proportion used is 30:30 mixed nanoparticles (CNPs) atpH 6.9. Additionally, Gompertz equation was adopted to fit the enhanced biohydrogen productions and Richard’s and Logistics equation were best suited for the biomass growth with minimal error. The batch kinetics of biohydrogen productions using nano catalysts were examined under batch fermentations. Thus, the addition of mixed nanoparticles showed significant yields in biohydrogen production for mixed nanoparticles and also be the promising method of fermentative yield of biohydrogen.
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