Abstract
A series of mehtods designed for electrophoresis of nuclear proteins is described. They deal with the low solubility of many nuclear proteins and with the presence of large amounts of nucleic acids. These were eliminated by enzymatic digestion, centrifugation, partition or precipitation. A combination of RNAse digestion and centrifugation is the method of choice when proteolysis is low. In the opposite case, precipitationor partition methods are preferred, at the expense of precipitation of some proteins. When the nuclear RNA content is low, centrifugation in an Airfuge is the simplest and the most efficient mehtod, superseding the widely used S1 nuclease method.
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