Abstract

For the first time a method is described for the cultivation of sufficient numbers of exoerythrocytic stages of a malarial parasite to facilitate the study of fine structure, the action of chemotherapeutic agents, the completion of timelapse cinemicrographic studies, and the more precise determination of growth rates and longevity. The method will be useful also for physiological studies, chemical analyses, and immunological studies on the extracellular, exoerythrocytic parasites. The use and modifications of commercially available media are described as well as alterations of procedures and techniques which meet the need for the production of exoerythrocytic stages in quantities not attained by other methods.

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