Abstract
A method is presented to monitor the chemical, chemiluminogenic and immunochemical properties of steroid N-(4-aminobutyl)- N-ethylsoluminol (ABEI) labels. The label preparation was applied to an isocratic reversed-phase HPLC system (with on-line ultraviolet detection) and fractionated into seventy 12-s fractions. In the fractions, the immunochemicla response was determined by radioimmunoassay and the chemiluminescence was determined in a luminometer or with photplate detection. From the results of the three detection techniques, conclusions can be drawn about the quality and purity of the label. The presence and identity of immunochemically or chemiluminogenically reactive decomposition products cn also be monitored. As an example, a preparation of 17β-testoterone-3-carboxymethyloxime/aminobutylethylisoluminol is analyzed. Possibilities for quality control, stability control and preparative purification are discussed.
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