Abstract
A procedure was developed to analyze phosphatidylcholine in ruminal protozoa and duodenal digesta, for the estimation of the flow of protozoal constituents from the stomach to the small intestine of ruminant animals. The procedure requires 15–20 mg of dry ruminal protozoa and 50–60 mg of dry duodenal digesta. Key words: Phosphatidylcholine, protozoa, duodenal flow, ruminants
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