Procalcitonin, Interleukin-1, Interleukin-10, Interleukin-13 and Tumor necrosis factor-alpha predictive value in the diagnosis of sepsis

Ansam Mohammad H,Zainalabdeen A Abdulla

doi:10.32894/kujss.2017.124939

Abstract

The present study is aimed to evaluate the role of some markers in the diagnosis of sepsis among SIRS (systemic inflammatory response syndrome) patients. The study included 180 patients with SIRS criteria within 24 hour of admission. They were admitted to different hospitals in Mosul city for the period from January 2013 to January 2014. From each patient two blood samples were collected, one for blood culture and the other separated by centrifugation and kept frozen for ELISA tests of PCT, IL-1, TNF-α, IL-10 and IL-13. IL-1, IL-10, IL-13 and PCT were having a significant difference in mean between culture positive and negative cases. PCT was significantly highly elevated in patients with sepsis (P=0.004) with high sensitivity (93.7%) and specificity (90.1%). Many combinations of markers were used in the current study to find out the best combination of markers. The results of using combined sensitivity and specificity of PCT together with 2 anti-inflammatory cytokines (IL-10 and IL-13) were the best. They were an excellent markers with 100% sensitivity and the highest specificity.

Highlights

Sepsis is a clinical term characterized by a marked attack upon the host by proinflammatory cytokines that has been precipitated by an infection
The traditional approach to sepsis diagnosis was based on clinical signs and symptoms of sepsis, such as fever, tachycardia and tachypnoea, supported by relevant microbiological data
Interleukin-1 (IL-1) is one of several pro-inflammatory cytokines produced during infection

Summary

Introduction

Sepsis is a clinical term characterized by a marked attack upon the host by proinflammatory cytokines that has been precipitated by an infection. Sepsis represents the body’s systemic response to severe infection. Sepsis leads to one or more severe complications for example hypotension, cardiac failure, coma, renal failure, intravascular coagulation. This phenomenon termed multiple organ dysfunction [1,2]. It is important to differentiate patients with sepsis from those with SIRS, as treatments may be very different [3]. Various attempts have been made to improve the diagnosis of infection. A recent prospective study supported the good predictive value of the IPS (Infection Probability Score) for a diagnosis of infection [6]. In sepsis an expanding number of cytokines have been found to be involved in the pathogenesis of the disease [7,8]

Methods

Results

Conclusion