Probiotic Proteinaceous Component(s) From Pseudomonas Fluorescence and Typhoid Immunity in Rabbits

Abstract

ABSTRACTA proteinaceous component(s) was (were) separated by polyethylene glycol 6000 (6%) from cell-free culture filtrates (CFCF) of Pseudomonas fluorescence. The protein concentration (Pc) was adjusted to 3 mg/ml. This Pc was tested as a probiotic component (PPC). To verity this possibility, six groups of local breed rabbits (O. caniculus) each of which of three rabbits were designated as I to VI. Groups III and IV were saline controls. Groups I and IV were vaccinated with heat killed S. typhi bacteria, while groups II and IV received heat killed S. typhi bacteria plus equal volumes of PPC from P. fluorescence. Groups I-III served for immunization program and groups IV to VI were used for S. typhi immunoprotection studies. Group II gave higher titers of antibodies against S. typhi “O”, a stronger skin DTH reaction and more LIF cytokines as compared to group I. Likewise, group VI demonstrated more potent immunoprotection than group V. Thus, PPC(s) could be considered to: 1) mediate DTH; 2) be humoral immune modulators; 3) enhance the release of LIF cytokines; 3) be potent immunoprotectants.