Probiotic Properties of Lactobacillus helveticus and Lactobacillus plantarum Isolated from Traditional Pakistani Yoghurt.

Mahreen Ul Hassan,Nuzhat Shafi,Nazia Akbar,Mike P Williamson,Madiha Khalid,Safira Attacha,Hina Nayab,Taghreed Saud Almansouri,Nasir Ali,Noreen Asim,Farheen Shafique,Maria E Potes

doi:10.1155/2020/8889198

Abstract

Probiotic bacteria are of utmost importance owing to their extensive utilisation in dairy products and in the prevention of various intestinal diseases. The objective of this study was to assess the probiotic properties of bacteriocin-producing isolates of Lactobacillus helveticus and Lactobacillus plantarum isolated from traditional Pakistani yoghurt. In this study, ten bacteriocin-producing isolates were selected to screen for the probiotic property. The isolates showed resistance to acidic pH (6-6.5), bile salt (0.01-1%), and 1-7% NaCl salt and showed good growth at acidic pH and antibacterial activity against ten different foodborne pathogens. Interestingly, these isolates were proved to be effective against Actinobacter baumannii but least effective against Klebsiella pneumoniae and Pseudomonas aeruginosa. A few isolates were found to be resistant to some antibiotics like vancomycim, gentamycin, erythromycin, streptomycin, and clindamycin. Our results provide strong evidence in favour of traditional Pakistani yoghurts as a potential source of bacteriocin-producing bacteria with an added benefit of the probiotic property. Specifically, LBh5 was considered a good probiotic isolate as compared to other isolates used in the study. Further extensive research should be done on isolation and characterisation of probiotic isolates from local fermented foods, and then, these isolates should be used in the development of probiotic enriched food supplements in Pakistan.

Highlights

Probiotic is a well-known term used for “live microorganisms” when added as supplements in food, which provide many health benefits
From 12 different isolates, five isolates were identified as L. helveticus, and of the rest five were identified as L. plantarum on the basis of their colony morphology and various biochemical tests, and the remaining two isolates were identified as E. coli and Enterococcus faecium
Bacteriocin-producing lactic acid bacteria were successfully isolated from traditional Pakistani yoghurt

Summary

Introduction

Probiotic is a well-known term used for “live microorganisms” when added as supplements in food, which provide many health benefits. The present study includes Lactobacillus, the probiotic genus popular for many medical applications such as decreasing enteric infections, intestinal tumors, and cholesterol levels, folate production, treating cardiovascular diseases, metabolic disorders, lactose intolerance, and boosting the immune system [5,6,7,8,9]. Such bacteria release anticarcinogenic and antimicrobial substances in addition to organic acids such as lactic acid, benzoic acid, and acetic acid [5, 10]. Lactobacillus plantarum is an indigenous gut inhabitant and is considered more compatible with the gut environment [12, 13]

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Conclusion