Probiotic properties of a phytase producing Pediococcus acidilactici strain SMVDUDB2 isolated from traditional fermented cheese product, Kalarei

Deepali Bhagat,Neelu Raina,Yugal Khajuria,Preeti Sharma,Parvez Singh Slathia,Meenu Katoch,Amit Kumar

doi:10.1038/s41598-020-58676-2

Deepali Bhagat, Neelu Raina + Show 5 more

Open Access

https://doi.org/10.1038/s41598-020-58676-2

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Abstract

The nutritional challenge faced by the monogastric animals due to the chelation effects of phytic acid, fuel the research on bioprospecting of probiotics for phytase production. Pediococcus acidilactici SMVDUDB2 isolated from Kalarei, exhibited extracellular phytase activity of 5.583 U/mL after statistical optimization of fermentation conditions viz. peptone (1.27%); temperature (37 °C); pH (6.26) and maltose (1.43%). The phytase enzyme possessed optimum pH and temperature of 5.5 and 37 °C, respectively and was thermostable at 60 °C. The enzyme was purified 6.42 fold with a specific activity of 245.12 U/mg with hydrophobic interaction chromatography. The purified enzyme had Km and Vmax values of 0.385 mM and 4.965 μmol/min respectively, with sodium phytate as substrate. The strain depicted more than 80% survival rate at low pH (pH 2.0, 3.0), high bile salt concentration (0.3 and 0.5%), after gastrointestinal transit, highest hydrophobicity affinity with ethyl acetate (33.33 ± 0%), autoaggregation (77.68 ± 0.68%) as well as coaggregation (73.57 ± 0.47%) with Staphylococcus aureus (MTCC 3160). The strain exhibited antimicrobial activity against Bacillus subtilis (MTCC 121), Mycobacterium smegmatis (MTCC 994), Staphylococcus aureus (MTCC 3160), Proteus vulgaris (MTCC 426), Escherichia coli (MTCC 1652) and Lactobacillus rhamnosus (MTCC 1408). The amount of exopolysaccharide produced by the strain was 2 g/L. This strain having the capability of phytate degradation and possessing probiotic traits could find application in food and feed sectors.

Highlights

The nutritional challenge faced by the monogastric animals due to the chelation effects of phytic acid, fuel the research on bioprospecting of probiotics for phytase production
In our study P. acidilactici SMVDUDB2 is capable of producing phytase, an exogenous enzyme suggesting potentially important mechanism of probiotic functionality and the ability to survive in gastrointestinal tract conditions; its adhesive properties and potential of producing EPS proves its wider industrial potential
Our observation reported the presence of mannose monomer in EPS from P. acidilactici SMVDUDB2 and absence of glucuronic acid or diacetyl ester monomeric unit

Summary

Introduction

The nutritional challenge faced by the monogastric animals due to the chelation effects of phytic acid, fuel the research on bioprospecting of probiotics for phytase production. KTU05-8 and KTU05-9 have shown highest extracellular phytase activity[7] report on production of exopolysaccharides (EPS) from these strains was absent.searching new strains of LAB with wider applications is an increasing need of the hour with traditional fermented foods of Southeast Asian countries which are less extensively explored, and some of the species from these sources could be of commercial potential[8]. The effect of EPS, a 2-substituted-(1-3)-β-D-glucan production was observed in case of Pediococcus damnosus (strain 2.6) with the change in concentration of glucose, temperature, and bacto casamino acids[11]. In our study P. acidilactici SMVDUDB2 is capable of producing phytase, an exogenous enzyme suggesting potentially important mechanism of probiotic functionality and the ability to survive in gastrointestinal tract conditions; its adhesive properties and potential of producing EPS proves its wider industrial potential

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